It's Gonna Be Bedlam

Bubonic Plague Released

Friday, June 19, 2009

Thirteen cases of Bubonic Plague have been recorded in Libya. Bubonic Plague is primarily a disease of rodents and their fleas, which can infect humans. It is transmitted between rodents by rodent fleas, and can be transmitted to people when infected rodent fleas bite them. It is a very severe disease in people, with case fatality rates of 50-60 percent if left untreated. A search for BPWMD vials have been in operation for over 12 years. Rumors of the Bubonic Plague being sent to Libya has been circulation for years.

A prime objective to create a weapon that could destroy millions has always been under the radar. Some bio-weapon teams has mastered the bicistronic operon yadBC (YPO1387 and YPO1388 of Y. pestis CO92; y2786 and y2785 of Y. pestis KIM5) in the Yersinia pestis strains. Adhesins of Yersinia pseudotuberculosis and Yersinia enterocolitica have been successful. In the 1970s and 1980 Libya had similiar cases of Y. pestis. Yersinia pestis is the etiological agent of plague, fatal in humans. Y. pestis can rapidly disseminate from a infection site into the lymphatic system and regional lymph nodes. The swelling of these infected lymph nodes into characteristic buboes is the classical symptom of bubonic plague. This disease can lead to colonization of a variety of tissues. Y. pestis becomes highly transmissible during coughing, and the bacteria can be easily inhaled, causing a primary pneumonic infection in a new host. Both systemic and pneumonic plague produces high mortality rates because of rapid proliferation of bacteria and quick onset of disease pathology.

This outbreak has prompted the Libyan government to call for an investigation of the cases by the World Health Organization (WHO). WHO will investigate deaths, not the Oca (oligomeric coiled-coil adhesins) or Vc family of proteins used as a subset of autotransporters. The putative promoter region of the Y. pestis yadBC operon can be amplified with primers P1 and P2 from SpeI-digested genomic DNA and cloned into KpnI/Acc65I-digested pBSlacZMCS to create a yadBC promoter-lacZ fusion giving you a pBS-PyadBC-lacZ, change that to the E. coli host S17-1, then conjugated into Y. pestis CO92.S1 to obtain strain CO92.S10 or into Y. pestis CO99-3015.S1 to obtain strain CO99-3015.S4.

This is a highly virulent pathogen because of its ability to escape the host immune system and rapidly proliferate within host tissues. It goes under the radar, E. coli pops up from the GST-YadB and GST-YadC antigens. WHO has to focus on putative structural analogs of YadA to find their lethality in this bubonic plague strains. It’s too difficult detecting YadB and YadC protein levels. Clone 4,146-bp fragments to Sall- and Sacl-digested pLD55, to create pLD55yadBD-L. Electroporate into Y. pestis CO92.S8 you should get a etracycline-sensitive isolation that contains both yadB and yadC. You can get YadBEcoRISD-52 and YadBPstl-32 and primers YadCEcoRI-52 and YadCHindlll-3. If done right you have a role in invasion, which is important for efficient trafficking to or colonization of lymphoid tissues in organs on infected sites.

Its Gonna Be BEDLAM!

FLU, A Weapon Of Mass Destruction

Wednesday, May 6, 2009

Influenza pandemics can claim million lives worldwide, Asiatic/Russian Flu claiming 1 million in 1889–90, Spanish Flu claiming 40 million in 1918–20, Asian Flu claiming 1.5 million 1957–58, Hong Kong Flu claiming 1 million in 1968–69 making influenza the perfect bitterer agent. Seasonal flu kills between 250,000 and 500,000 people globally in an average year.

For years we searched for Weapons Of Mass Destruction thinking it was huge canisters of urania-yellowcake, anthrax, chemical gases, when in fact it small, it’s viruses. Once released, the virus would not discriminate between friend or foe. Biological Warfare is nothing new, it’s been used for years AIDS, SARS, Ebola, and West Nile Virus. With new DNA technology, researchers can construct viruses and bacteria that can jump the species barrier, bred to spread. Flu viruses mutate constantly, which is why the flu vaccine is changed every year, and they can also swap DNA in a process called reassortment.

Biotechnical breakthrough has deciphered a way of reconstructing the chloroplast signal peptide, hydrophobic region, coil motif and TPR region with a basic helix-loop-helix to prevent protein biosynthesis on the outer surface controlling the glutamyl-tRNA reductase (GLU-TR) by heme implicated in protein–protein interaction. The FLU protein operates independently of heme making conventional vaccines useless. This reverse genetic engineered strain is highly transmissible and transmits well from person to person. This DNA becomes part of the cell, giving it the blueprint it needs to build. Scientists create DNA vaccines by into a circular loop called a plasmid. DNA injected into muscle cells reproduce the viral coat protein and trigger immunization reverse that and you have a weapon. Manmade strains of influenza capable of triggering a human flu pandemic is called "passaging" and involves the repeated cycling of strains of a virus through cell culture.

Pathologist has genetically reconstructed the 1918 Spanish influenza strain (H1N1), one of most deadliest viruses known to humankind. Nine fragments of Spanish flu viral RNA were revealed. Four of the eight viral RNA segments has been completely sequenced including the genes for hemagglutinin (HA) and neuraminidase (NA). H1N1 killed an estimated 20-40 million people worldwide. The virus is an influenza A, H1N1 and contains DNA typical to avian, swine and human viruses, this molecular techniques could be used for the purpose of bioterrorism and when it acquires the ability to pass from person to person, it can spark a pandemic.

Its - Gonna - Be - BEDLAM